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Human monocytes express Wnt signaling <t>components</t> and the Wnt-3a ligand is found in the plasma. A Analysis of single-cell RNA-seq data by 10X genomics, based on a Seurat tutorial (“pbmc3k”). tSNE plot of the data with annotation of cell types (upper left panel). Classical monocytes are the orange cluster labeled “CD14 + Mono” and non-classical monocytes are the light blue cluster labeled “CD16 + Mono”. Plt – platelets. NK – natural killer cells. B and T lymphocytes are abbreviated B and T, respectively. The monocyte cluster (red circle) was further analyzed for expression of Wnt signaling components. The genes are grouped by functional categories (color-coded at the bottom). B Wnt-3a levels were measured by ELISA in isolated human plasma obtained from multiple healthy donors. Protein concentrations were calculated by regression from a standard curve
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Human monocytes express Wnt signaling <t>components</t> and the Wnt-3a ligand is found in the plasma. A Analysis of single-cell RNA-seq data by 10X genomics, based on a Seurat tutorial (“pbmc3k”). tSNE plot of the data with annotation of cell types (upper left panel). Classical monocytes are the orange cluster labeled “CD14 + Mono” and non-classical monocytes are the light blue cluster labeled “CD16 + Mono”. Plt – platelets. NK – natural killer cells. B and T lymphocytes are abbreviated B and T, respectively. The monocyte cluster (red circle) was further analyzed for expression of Wnt signaling components. The genes are grouped by functional categories (color-coded at the bottom). B Wnt-3a levels were measured by ELISA in isolated human plasma obtained from multiple healthy donors. Protein concentrations were calculated by regression from a standard curve
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Human monocytes express Wnt signaling components and the Wnt-3a ligand is found in the plasma. A Analysis of single-cell RNA-seq data by 10X genomics, based on a Seurat tutorial (“pbmc3k”). tSNE plot of the data with annotation of cell types (upper left panel). Classical monocytes are the orange cluster labeled “CD14 + Mono” and non-classical monocytes are the light blue cluster labeled “CD16 + Mono”. Plt – platelets. NK – natural killer cells. B and T lymphocytes are abbreviated B and T, respectively. The monocyte cluster (red circle) was further analyzed for expression of Wnt signaling components. The genes are grouped by functional categories (color-coded at the bottom). B Wnt-3a levels were measured by ELISA in isolated human plasma obtained from multiple healthy donors. Protein concentrations were calculated by regression from a standard curve

Journal: Cell Communication and Signaling : CCS

Article Title: Wnt signaling regulates chemokine production and cell migration of circulating human monocytes

doi: 10.1186/s12964-024-01608-8

Figure Lengend Snippet: Human monocytes express Wnt signaling components and the Wnt-3a ligand is found in the plasma. A Analysis of single-cell RNA-seq data by 10X genomics, based on a Seurat tutorial (“pbmc3k”). tSNE plot of the data with annotation of cell types (upper left panel). Classical monocytes are the orange cluster labeled “CD14 + Mono” and non-classical monocytes are the light blue cluster labeled “CD16 + Mono”. Plt – platelets. NK – natural killer cells. B and T lymphocytes are abbreviated B and T, respectively. The monocyte cluster (red circle) was further analyzed for expression of Wnt signaling components. The genes are grouped by functional categories (color-coded at the bottom). B Wnt-3a levels were measured by ELISA in isolated human plasma obtained from multiple healthy donors. Protein concentrations were calculated by regression from a standard curve

Article Snippet: Canonical Wnt signaling components are expressed in monocytes (data by 10X Genomics).

Techniques: Clinical Proteomics, RNA Sequencing, Labeling, Expressing, Functional Assay, Enzyme-linked Immunosorbent Assay, Isolation